Forensics

Scientist preparing a slide on a microscope. ATF laboratories are staffed with highly trained chemists, forensic biologists, engineers, fingerprint specialists, firearm and tool mark examiners, document examiners and administrative personnel. Many of these professionals have held leadership positions in numerous professional scientific organizations and are among the most highly qualified specialists in their fields.

Publications

The below documents are management system documents utilized by ATF Laboratories. They are provided for informational purposes only. Sensitive or copyrighted information has been redacted. The documents are used in ATF Laboratories and not published with the intent of setting a policy or analysis standard for other laboratories. The inclusion of equipment/software manufacturer names does not imply endorsement by ATF Laboratories.

The documents were current as of February 28, 2018. ATF management system documents are reviewed annually and revised as needed. For specific requests, submit a Freedom of Information Act (FOIA) request. Instructions on how to file a FOIA request are found at: www.atf.gov/resource-center/freedom-information-act-foia.

Name Description Type
ATF-LS-FB02 Alternate Light Source (252.1 KB)
An Alternate Light Source (ALS) utilizes specific wavelengths of light to help locate body fluid stains on clothing or other items of evidence.
Forensic Biology
ATF-LS-FB05 Phenolphthalein Test for Blood (283.94 KB)
This protocol is to be used when examining an exhibit of evidence for the presence of blood and is only a presumptive test.
Forensic Biology
ATF-LS-FB08 General QIAamp Micro DNA Extraction (330.63 KB)
This protocol can be used to extract and purify DNA from reference samples and evidence samples containing biological material.
Forensic Biology
ATF-LS-FB09 General QIAamp Investigator DNA Extraction (339.33 KB)
This protocol is to be used to extract and purify DNA from reference samples and samples of evidence containing biological material.
Forensic Biology
ATF-LS-FB11 Quantifiler (1.75 MB)
This protocol is to be used to estimate the concentration of DNA in an extract.
Forensic Biology
ATF-LS-FB12 30 kD Microcon® DNA Concentration / Purification (275.83 KB)
This protocol is used to concentrate and/or further purify DNA extracts.
Forensic Biology
ATF-LS-FB14 Identifiler (335.13 KB)
This protocol is to be used with the Applied Biosystems (AB) AmpFlSTR™ Identifiler™ PCR Amplification Kit to amplify DNA extracted from known reference samples or biological material found on items of evidence.
Forensic Biology
ATF-LS-FB15 Applied Biosystems™ 3130 Genetic Analyzer (606.6 KB)
This protocol is used to analyze the amplified DNA product on the Applied Biosystems™ (AB) 3130 Genetic Analyzer.
Forensic Biology
ATF-LS-FB16 Applied Biosystems GeneMapper ID (333.17 KB)
This protocol is used to analyze the raw data generated by the Applied Biosystems™ (AB) 3130 Genetic Analyzer.
Forensic Biology
ATF-LS-FB17 Identifiler STR Interpretation Guidelines (1.47 MB)
The interpretation guidelines are intended to be a general guide for the evaluation of the typing results for DNA amplified with the AmpFlSTR™ Identifiler™ PCR Amplification Kit and analyzed with the Applied Biosystems (AB) 3130 Genetic Analyzer and GeneMapper® ID v3.2 analysis software.
Forensic Biology
ATF-LS-FB18 Forensic Biology Report Wording: Reporting the Results of Forensic Biology Analysis (357.56 KB)
The following guidelines outline the wording to be used when reporting the results of Forensic Biology analyses.
Forensic Biology
ATF-LS-FB20 Forensic Biology Case Note Glossary (236.61 KB)
This glossary is intended for use as a guide for members of the ATF Forensic Science Laboratory DNA Sections. It is not intended to be all-inclusive.
Forensic Biology
ATF-LS-FB21 Swabbing Evidence for DNA Analysis (375.19 KB)
This method describes how to sample evidence items for potential biological material suitable forDNA analysis.
Forensic Biology
ATF-LS-FB22 Staff DNA Index (297.52 KB)
The purpose of the DNA Staff Index is to detect contamination of DNA profiles resulting from individuals who handled the evidence during, or after, the investigation or those who may enter evidence processing laboratories during the course of routine duties.
Forensic Biology
ATF-LS-FB23 Low Template DNA Extraction using the QIAamp® Investigator Kit on the QIAcube® Robotic Workstation (1.9 MB)
This protocol is used to extract and purify DNA from low template evidence samples and hair root material.
Forensic Biology
ATF-LS-FB26 Outsourced Casework Samples (259.34 KB)
This procedure covers outsourcing of casework samples and ensures that a technical review is performed to verify the integrity of the DNA data received from the vendor laboratory.
Forensic Biology
ATF-LS-FB27 BlueStar Presumptive Test for Blood (261.12 KB)
This protocol is to be used when examining an exhibit of evidence or a crime scene for the presence of blood and is only a presumptive test.
Forensic Biology
ATF-LS-FB28 Quality Control of Reagents (466.41 KB)
This protocol outlines the frequency and type of quality control checks that are to be conducted on critical reagents to ensure the high quality of work suitable for a forensic casework laboratory.
Forensic Biology
ATF-LS-FB29 Quality Control of Instruments and Equipment Maintenance (589.92 KB)
This protocol outlines the frequency and type of maintenance, calibration, and/or verifications that are to be conducted to keep our instruments and equipment in good working order and suitable for use in forensic casework.
Forensic Biology
ATF-LS-FB30 GlobalFiler™ PCR Amplification Kit (546.04 KB)
This protocol is to be used to amplify DNA extracted from known reference samples or biological material found on items of evidence.
Forensic Biology
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