Laboratory Resources

The below documents are management system documents utilized by ATF Laboratories. They are provided for informational purposes only. Sensitive or copyrighted information has been redacted. The documents are used in ATF Laboratories and not published with the intent of setting a policy or analysis standard for other laboratories. The inclusion of equipment/software manufacturer names does not imply endorsement by ATF Laboratories.
 
The documents were current as of February 28, 2018. ATF management system documents are reviewed annually and revised as needed. For specific requests, submit a Freedom of Information Act (FOIA) request. Instructions on how to file a FOIA request are found at: www.atf.gov/resource-center/freedom-information-act-foia.
Name Description Type
ATF-LS-FB31 Applied Biosystems™ 3130 Genetic Analyzer -GlobalFiler™ (554.2 KB)
This protocol is used to analyze the amplified DNA product on the Applied Biosystems™ (AB) 3130 Genetic Analyzer.
Forensic Biology
ATF-LS-FB32 Applied Biosystems™ GeneMapper® ID-X Software (2.08 MB)
This protocol is to be used to analyze the raw data generated by the Applied Biosystems™ (AB) 3130 Genetic Analyzer.
Forensic Biology
ATF-LS-FB35 General QIAamp Micro Double AL DNA Extraction (337 KB)
This protocol is to be used to extract and purify DNA from reference samples and samples of evidence containing biological material.
Forensic Biology
ATF-LS-FD - Introduction (227.79 KB)
The ATF Laboratories’ Quality Program includes technical operational guidelines for each discipline area. These guidelines were established by ATF laboratory employees, specific to each discipline.
Fire Debris
ATF-LS-FD - Standard Approach to Liquid Samples (272.8 KB)

This document is a flow chart depicting the fire debris analysis scheme (testing order) for testing neat liquids to determine if the liquid is ignitable/flammable.

Fire Debris
ATF-LS-FD 1 - Gas Chromatography-Mass Spectrometry (GC-MS) (318.82 KB)
This policy and procedure guideline establishes a standard method for identification and classification of ignitable liquids and their residues extracted from fire debris by Gas Chromatography-Mass Spectrometry (GC-MS).
Fire Debris
ATF-LS-FD 2 - High-Temperature Gas Chromatography-Mass Spectrometry Analysis (284.81 KB)
This policy and procedure guideline establishes a standard method for identification and classification of materials by High Temperature Gas Chromatography-Mass Spectrometry (HTGC-MS). This method is useful for the analysis of oils, waxes, lubricants (such as petroleum jelly), and plasticizers.
Fire Debris
ATF-LS-FD 3 - Simple Headspace (252.52 KB)
This policy and procedure guideline establishes a standard method for sampling headspace vapors from fire debris evidence.
Fire Debris
ATF-LS-FD 4 - Passive Charcoal Adsorption (286.71 KB)
This policy and procedure guideline establishes a standard system for extracting fire debris evidence by the passive headspace concentration method (activated charcoal strip).
Fire Debris
ATF-LS-FD 5 - Dynamic Charcoal Adsorption (285.24 KB)
This policy and procedure guideline establishes a standard procedure for extracting fire debris evidence by the dynamic headspace concentration method (dynamic charcoal adsorption).
Fire Debris
ATF-LS-FD 6 - Solvent Extraction (253.32 KB)
This policy and procedure guideline establishes a standard method for separating small quantities of ignitable liquid residues from fire debris samples or aqueous liquids.
Fire Debris
ATF-LS-FD 7 - Ignitable Liquid Reference Library (244.68 KB)
This policy and procedure establishes the maintenance of an Ignitable Liquid Reference Library.
Fire Debris
ATF-LS-FD 8 - Ignitable Liquid Classification System (307.88 KB)
This policy and procedure guideline establishes a standard Ignitable Liquid Classification System.
Fire Debris
ATF-LS-FD 9 - Report Wording (317.45 KB)
This policy and procedure guideline establishes examples that may be used in laboratory reports. Report wording will accurately reflect what was identified in the sample and clearly communicate those findings to the reader.
Fire Debris
ATF-LS-FD10 - Extraction and Analysis of Vegetable Oils (319.44 KB)
This policy and procedure guideline establishes a standard method for extracting, derivatizing, and analyzing vegetable oils encountered in neat liquids and fire debris samples.
Fire Debris
ATF-LS-FD12 - Ignition Susceptibility (237.45 KB)
Initial testing/screening of unknown liquids is performed to determine if they will support combustion.
Fire Debris
ATF-LS-FD13 - Fire Debris Glossary (246.75 KB)
This glossary is intended for use as a guide for ATF Forensic Science Laboratory Fire Debris Chemists.
Fire Debris
ATF-LS-FD14 - Analysis of Waxes and Petrolatum (289.05 KB)
This policy and procedure guideline establishes a standard method for the analysis and identification of waxes and petrolatum encountered in evidentiary samples.
Fire Debris
ATF-LS-FD15 - Analysis of Lubricating Oils (283.31 KB)
This policy and procedure guideline establishes a standard method for extracting and analyzing lubricating oils encountered in neat liquids and debris samples.
Fire Debris
ATF-LS-FRL 1588 Thermocouple Standard Operating Procedures (120.33 KB)
Standard operating procedures for 1588 thermocouple. 
Fire Research Engineer Testing
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